They don’t necessary have to be colored compounds, since there are many other ways to detect or identify them. It was a technique to separate substances that had different colors.īasically, a chromatography is any lab technique in which we separate different chemical components of a mixture by their affinity to a stationary phase (usually silica gel in TLC) and to a mobile phase (the solvent or mixture of solvents). The word chromatography comes from the Greek chroma, “color”, and graphein, “to write”. You might be familiar with what chromatography is, but maybe you din’t know that, as a matter of fact, the name “chromatography” comes from some early experiments on thin layer chromatography.
Happy TLCing everyone!ġ0 Closing Up and Conclusions What is Thin Layer Chromatography? On the other hand, I can also promise you that even if you have PhD in organic synthesis, there is still some tricks or hacks to learn in this guide.Ĭonsidering this, you can navigate this tutorial page by using the index shown right below. I can tell you that even if you have never been in a chemistry lab before, you will be prepared to do a thin layer chromatography just by continuing to read the first sections. What I decided to do, is to put together all my knowledge in this tutorial article, so you can start reading without knowing what a TLC is, and finish up by being able to separate and identify (almost) anything you want in an organic chemistry lab! You could say that there are very few things that I still don’t know about this technique.
I also enjoy sharing and reading lab tricks with colleagues, or even online. Well, I am a synthetic organic chemist with years of experience in the lab, and I have run thousands of TLC and flash columns in any solvent combination that you can imagine. So, what is this tutorial about? What am I going to learn if I continue reading?
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